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bypassing the pluripotent stem cell stage May 2

SuChun Zhang center, professor of neuroscience in the School of Medicine and Public Health, talks with postdoctoral student Lin Yao in his research lab at the Waismam Center.

Photo: Jeff Miller

A UWMadison research group has converted skin cells from people and monkeys into a cell that can form a wide variety of nervoussystem cells without passing through the doitall stage called the induced pluripotent stem cell, or iPSC. IPSC cells can generate any cell type, which could be a problem for cellbased therapy to repair damage due to disease or injury in the nervous system.

In particular, the absence of iPSC cells rules out the formation of tumors by pluripotent cells in the recipient, a major concern involving stem cell therapy.

A second advance comes from the virus that delivers genes to reprogram the adult skin cells into a different and more flexible form. Unlike other viruses used for this process, the Sendai virus does not become part of the cells genes.

Progenitor cells grown from the skin of ALS or spinal muscular atrophy patients can be transformed into various neural cells to model each disease and allow rapid drug screening. . Eventually, the process could produce cells used to treat conditions like spinal cord injury and ALS.

Jianfeng Lu, Zhangs postdoctoral research associate at the UWMadison Waisman Center, removed skin cells from monkeys and people, and exposed them to Sendai virus for 24 hours. Lu then warmed the culture dish to kill the virus without harming the transforming cells. Thirteen days later, Lu was able to harvest a stem cell called an induced neural progenitor. After the progenitor was implanted into newborn mice, neural cells seemed to grow normally, without forming obvious defects or tumors, Zhang says.

Other researchers have bypassed the pluripotent stem cell stage while turning skin cells into neurons and other specialized cells, Zhang acknowledges, but the new research, just published in Cell Reports, had a different goal. Our idea was to turn skin cells to neural progenitors, cells that can produce cells relating to the neural tissue. These progenitors can be propagated in large numbers.

The research overcomes limitations of previous efforts, Zhang says. First, the Sendai virus, a kind of cold virus, is considered safe because it does not enter the cells DNA, and it is killed by heat within 24 hours. This is quite similar to the fever that raises our temperature to remove cold virus. Second, the neural progenitors have a greater ability to grow daughter cells for research or therapy. Third, the progenitor cells are already well along the path toward specialization, and cannot become, say, liver or muscle cells after implantation. Finally, the progenitors can produce many more specialized cells.

The neurons that grew from the progenitor had the markings of neurons found in the rear of the brain, and that specialization can also be helpful. For therapeutic use, it is essential to use specific types of neural progenitors, says Zhang. We need regionspecific and functionspecific neuronal types for specific neurological diseases.

Progenitor cells grown from the skin of ALS Lou Gehrigs disease or spinal muscular atrophy patients can be transformed into various neural cells to model each disease and allow rapid drug screening, Zhang adds.

Eventually, the process could produce cells used to treat conditions like spinal cord injury and ALS..

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Sapphire Radeon HD 5850 Toxic 1GB Video Card Review

Last month Sapphire released the Radeon HD 5850 Toxic, which is a custom designed ATI Radeon HD 5850 that looks nothing like the reference designed board that it is derived from. The Sapphire Toxic Radeon HD 5850 1GB card that we have here today is clocked at 765MHz on the core and 1125MHz 4500MHz effective on the memory, which gives it a significant boost in clock speed over the reference designs 700MHz core clock and 1000MHz memory clock speeds. The card also comes with Sapphires VaporX technology that should allow the card to run 15 degrees C cooler and 10dB quieter than the standard models, while providing additional headroom for even higher overclocking.

The Sapphire HD 5850 TOXIC Edition is a Sapphire is just like any other Radeon HD 5850 graphics card and has 1440 stream processors and 72 texture units that need to be kept cool. Sapphire has kept the card nice and cool thanks to the custom three heatpipe heatsink and the large eleven blade cooling fan that is placed directly above the GPU core. The fan shroud on the Sapphire HD 5850 TOXIC Edition is nice looking and is also a one off piece that was designed just for this card. It should be noted that this fan is a two ball bearing model and is rather quiet for a video card!

Flipping the Sapphire HD 5850 TOXIC Edition video card over you can get a better look at the back, and if you look closely youll see that the entire GPU cooling solution is held on by the four silver Philips screws around the GPU. The two black Philips screws on the left side are used to hold on the passive cooler for the voltage regulators. In case you are wondering, Sapphire did use their Black Diamond chokes on this card again, which is said to help lower operating temperatures and increase the efficiency of the chokes in general by up to 25%.

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Charges Filed In MSO Singer Murder

MILWAUKEE Charges were filed Wednesday in the murder of a singer with the Milwaukee Symphony Orchestra.

The criminal complaint says Gerhard Witte admits he slashed his exwifes throat.

According to the criminal complaint, 70yearold Gerhard Witte told police he went to see his exwife perform at the Milwaukee Symphony Orchestra last weekend. Later, he waited for her in the parking structure.

Elisabeth Witte left a bar after celebrating the performance with friends. They saw her exhusband and called police, knowing she had a restraining order against him. Friends heard Elisabeth Witte screaming and tried to get her in an elevator, but it closed.

Police say thats when Witte stabbed his exwife repeatedly. When officers got there, they say they saw Gerhard Witte kneeling over his exwife in a pool of blood.

The complaint says he told them: Shes dead. I killed her. Im a doctor, so I know.

The complaint goes on to say he later told police he was upset about their divorce and said, Listen, Im a party to murder, which involves the court system and Im never going to leave prison. Im going to die in here. My life is over, my family is ruined.

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Area man accused of fraudulently claiming ownership of unoccupied homes

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A local man is accused of fraudulently claiming ownership of two unoccupied homes one in Olympia, the other in Yelm by filing of adverse possession in the Thurston County Auditor Office. He is accused of going to the homes, changing the locks, and trying to rent one of them to a woman who lost $500 after signing a fraudulent rental agreement with him, court papers state.

Lee Van Nguyen, 31, has pleaded not guilty in Thurston County Superior Court to charges of residential burglary, attempted firstdegree theft and obtaining a signature by deception. He is out of custody after posting bail. Nguyen attorney, Alexander Frix, declined to comment on the pending case when reached by telephone Thursday.

An employee in the Thurston County Auditor Office, Heather Hirotaka, confirmed that Nguyen recorded two affidavits of adverse possession on March 21 for the two properties. But she said that just because her office records a document, that doesn make it legally binding. Hirotaka added that in her 14plus years in the auditor office, is the first time I seen an affidavit of adverse possession.

Thurston County Chief Civil Deputy David Klumpp said there is a legal process for claiming a property under adverse possession. However, Klumpp said, a person has to possess or use the property for at least 10 years before filing an adverse possession claim. A person making an adverse possession claim on a property must also pay the taxes on the property, he said.

Also, Klumpp said, owner should know that you attempting to adversely possess the property, which he added, does not appear to be the case in Nguyen situation.

Thurston County Sheriff deputies arrested Nguyen on April 2, after a realestate agent reported a suspicious person inside a residence she was selling in the 3400 block of Forrest Parse Lane in Olympia. The agent said she was in the process of selling the home to a buyer for $615,000.

Sheriff deputies were dispatched to the residence, and Nguyen gave them an affidavit of adverse possession, which he claimed were papers showing that he owned the property. woman in the rental agreement with Nguyen was sleeping at the property when sheriff deputies arrived. She also had moved her belongings there. She told deputies she paid Nguyen $500 and signed a rental agreement. She was upset and had to call someone to move her belongings out.

The same day, sheriff deputies learned Nguyen also had filed the adverse possession affidavit for a home on the 15000 block of 100th Avenue in Yelm. He had changed the locks at the unoccupied home, and had gone around the neighborhood identifying himself as the new owner. A deputy learned that the real owner is a service member stationed in Alaska. The owner said he was selling the home but had not sold it to Nguyen or given him permission to be there.

In an unrelated case, Nguyen has a pending felony charge of malicious mischief for allegedly kicking and breaking a door and shattering ornate glass at Fujiyama Japanese Steakhouse in Olympia. According to court papers, he was angry because of an automatic gratuity on his bill..

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Background noise on youtube videos

I dont know if this is the right place to post this, sorry if it isnt.

So, basicly whenever im viewing a video on youtube expecificly music videos my headphones start going crazy and doing alot of background white noise i think thats what its called whenever there is a strong bass sound, as if they were broken.

The problem is, this only happens when viewing said videos on youtube. Ive tried downloading a couple and listenning to them while not on youtube and they dont give me any problems at all, same goes for similar videos on other websites such as vimeo.

Ive also tried with other headphones and nothing changes.

The only thing ive installed lately and I think this started happening after the instalation and a computer reset was the AMD Catalyst Control Centre, which I have no idea how it could possibly affect sound. Looked around in the CCC control panel and there is nothing related to sound.

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Russian forces will provide security at events in the US

The Ministry of the Russian Federation put out a press release on June 26th which explained a new deal that has been signed between the Russian Emergency Situations Ministry and the United States Federal Emergency Management Agency. FEMA

According to the new deal, Russia and the United States will exchange experts during joint rescue operations and in major disasters and exchange experience in monitoring and forecasting emergency situations, training rescuers, developing minerescuing and providing security at mass events.

So according to this deal, Russian forces will be present at any event considered a National Special Security Event, which, according to the United States Secret Service website, is a title designated by the Secretary of Homeland Security. Past events that have been considered National Special Security Events include Super Bowls, State of the Union Addresses, the Olympics, and the Democratic and Republican national conventions.

United States and Russian forces have previously trained together on US soil. In fact, the first time Russians ever trained on US soil was just last year.